Activation of the cardiac mTOR/p70 pathway by leucine requires PDK1 and correlates with PRAS40 phosphorylation
نویسندگان
چکیده
Cossette Sanchez Canedo, Bénédicte Demeulder, Audrey Ginion, Jose R. Bayascas, Jean-Luc Balligand, Dario R. Alessi, Jean-Louis Vanoverschelde, Christophe Beauloye, Louis Hue, and Luc Bertrand Division of Cardiology, Unit of Pharmacology and Therapeutics, Hormone and Metabolic Research Unit and de Duve Institute, Université catholique de Louvain, Brussels, Belgium; and MRC Protein Phosphorylation Unit, University of Dundee, Dundee, United Kingdom
منابع مشابه
Activation of the cardiac mTOR/p70(S6K) pathway by leucine requires PDK1 and correlates with PRAS40 phosphorylation.
Like insulin, leucine stimulates the mammalian target of rapamycin (mTOR)/p70 ribosomal S6 kinase (p70(S6K)) axis in various organs. Insulin proceeds via the canonical association of phosphatidylinositol 3-kinase (PI3K), phosphoinositide-dependent protein kinase-1 (PDK1), and protein kinase B (PKB/Akt). The signaling involved in leucine effect, although known to implicate a PI3K mechanism indep...
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p70 ribosomal protein S6 kinase 1 (S6K1) is regulated by multiple phosphorylation events. Three of these sites are highly conserved among AGC kinases (cAMP dependent Protein Kinase, cGMP dependent Protein Kinase, and Protein Kinase C subfamily): the activation loop in the kinase domain, and two C-terminal sites, the turn motif and the hydrophobic motif. The common dogma has been that phosphoryl...
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BACKGROUND The p70 S6 kinase, an enzyme critical for cell-cycle progression through the G1 phase, is activated in vivo by insulin and mitogens through coordinate phosphorylation at multiple sites, regulated by signaling pathways, some of which depend on and some of which are independent of phosphoinositide 3-kinase (Pl 3-kinase). It is not known which protein kinases phosphorylate and activate ...
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Thyroid hormones affect cardiac growth and phenotype; however, the mechanisms by which the hormones induce cardiomyocyte hypertrophy remain uncharacterized. Tri-iodo-L-thyronine (T3) treatment of cultured cardiomyocytes for 24 h resulted in a 41 +/- 5% (p < 0.001) increase in [(3)H]leucine incorporation into total cellular protein. This response was abrogated by the phosphatidylinositol 3-kinas...
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